Katharine ME
Katharine ME
  • Видео 8
  • Просмотров 120 051

Видео

Which DNA test is best? Whole Genome Sequencing, Whole Exome Sequencing, and Genotyping - EXPLAINED
Просмотров 14 тыс.2 года назад
Trying to decide on what kind of DNA test to get? Confused over the many consumer offerings and about what your doctor is telling you? This video contains everything you need to know. Here I compare whole genome sequencing, whole exome sequencing, and genotyping on: how much of the genome they cover, how they work, their cost, and what they can tell us. 00:00 Intro 00:30 Genomics review 01:55 G...
FASTQ, BAM, and VCF file formats easily explained - A must watch if you have had a DNA test
Просмотров 15 тыс.2 года назад
FASTQ file format, BAM file format, SAM file format, and VCF file format explained simply for a person with no scientific or technical background. If you have had a DNA test, this video is a must watch for you. You do not need to let your precious genome files sit untouched and unused on a hard drive. This is the first video in a series where I will help you understand and make use of these fil...
DNA Sample Collection Methods - the BEST and the WORST
Просмотров 4,1 тыс.2 года назад
A must watch before you order a DNA test! Here I compare three methods for DNA collection: blood, cheek swab, and saliva. Each are commonly used in the clinic and for consumer DNA tests, but they are not created equal! Learn about the pros and cons of each. 00:00 Intro 00:29 What makes a great DNA sample 02:53 BLOOD 04:16 SWAB 05:53 SALIVA 07:25 Conclusion 07:30 Which method companies use
VCF File Format Explained | General Structure & Columns
Просмотров 13 тыс.3 года назад
This video is a great starting point or review of the VCF file format. Its evolving so be sure to check Samtools' hts-specs repository for the latest: github.com/samtools/hts-specs You may recognize this video in the IGV channel's VCF Basics video, and thats because I made the IGV VCF Basics video, as well as all the other IGV videos currently on that channel.
QTL Analysis Explanation and Example
Просмотров 71 тыс.7 лет назад
In this video I describe how QTL Analysis (Quantitative Trait Loci Analysis) works and give an example of how it could be used to study a particular trait. QTL mapping QTL analysis Quantitative Trait Loci Analysis

Комментарии

  • @CeiolKeeils-e4b
    @CeiolKeeils-e4b 15 часов назад

    Lewis Dorothy Davis Sharon Martin Charles

  • @stephenjohnson9733
    @stephenjohnson9733 День назад

    good explanation thanks

  • @JuliaAmos-i7e
    @JuliaAmos-i7e День назад

    Wilson Daniel Davis Ronald Williams James

  • @DolaAktar-t9d
    @DolaAktar-t9d 2 дня назад

    Jackson Michael Brown Ruth Rodriguez Anthony

  • @WilliamNordstrom-l6s
    @WilliamNordstrom-l6s 2 дня назад

    Jackson Lisa Johnson Sharon Martin Timothy

  • @PamellaGurtin-k9r
    @PamellaGurtin-k9r 3 дня назад

    Harvey Landing

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    @MaryannHart-z4q 3 дня назад

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    @HarlandJanusz-p9p 4 дня назад

    Stephanie Unions

  • @JerryBeamer-g8m
    @JerryBeamer-g8m 4 дня назад

    Toni Curve

  • @secondeye3927
    @secondeye3927 4 дня назад

    thank you very much. this is so helpful and very clear to understand easily

  • @SandraWilliams-q7u
    @SandraWilliams-q7u 4 дня назад

    Block Stravenue

  • @MelvilleKenneth-j9z
    @MelvilleKenneth-j9z 5 дней назад

    Bartoletti Curve

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    @BronteChrist-b7v 5 дней назад

    Roob Squares

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    @RohitSormaes 5 дней назад

    Frieda Row

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    @ChristineOshiro-z2s 5 дней назад

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    @HodgsonBennett-e6u 5 дней назад

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    @SearchRreon-z3n 5 дней назад

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    @HamletRiva-k6j 5 дней назад

    Wintheiser Squares

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    @OscarAlbert-h9f 5 дней назад

    Koepp Dale

  • @MrFilu13
    @MrFilu13 5 дней назад

    Good... 👍 Nicely explain ed

  • @VernettaDain-q6r
    @VernettaDain-q6r 6 дней назад

    Ernser Island

  • @ChayaLiechti-w3w
    @ChayaLiechti-w3w 6 дней назад

    Lavern Roads

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    @PeggyVenus-j7p 6 дней назад

    Borer Orchard

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    @HoltDinah-p6o 6 дней назад

    Rohan Highway

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    @WesleyRock-m5u 6 дней назад

    Brakus Stravenue

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    @VernaCooley-h9i 7 дней назад

    Paucek Drive

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    @BnmTyu-p5v 7 дней назад

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    @nicolefaneers1028 7 дней назад

    O'Hara Drive

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    @user-dg2nd8tg7z 7 дней назад

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    @EvanNimmo-n5w 7 дней назад

    Kristofer Village

  • @DeneseZana-z2n
    @DeneseZana-z2n 7 дней назад

    Clovis Track

  • @ZackarySmith-x2j
    @ZackarySmith-x2j 7 дней назад

    Hall Gary Moore Shirley Young Maria

  • @JaneJuliet-m9x
    @JaneJuliet-m9x 8 дней назад

    Smith Kimberly Moore Michelle Clark Matthew

  • @JonathanVanier-f6k
    @JonathanVanier-f6k 8 дней назад

    Mathias Square

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    @MorseHunter-p7m 8 дней назад

    Lopez Nancy Young Mary Brown Daniel

  • @WsdcteHsdgxhte
    @WsdcteHsdgxhte 9 дней назад

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    @DonkpApmhp-n7p 9 дней назад

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  • @arioche
    @arioche 12 дней назад

    great

  • @AmandaGarcia-j9q
    @AmandaGarcia-j9q 13 дней назад

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  • @JakirHossene
    @JakirHossene 17 дней назад

    Young Patricia Garcia Carol Harris Michael

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    @EricLee-p5w 18 дней назад

    Thomas Gary Martin Christopher Miller Ronald

  • @europhile2658
    @europhile2658 Месяц назад

    excellent description!

  • @reality-winner5759
    @reality-winner5759 Месяц назад

    If you still don't believe in a creator at this point, you're a moron

  • @md82892
    @md82892 2 месяца назад

    I bought a wgs and the VCF file company supplied is missing a lot of SNP's that are critical and common. I asked the company that how come if they're doing 30x sequencing of 100% of DNA these common SNP's are missing, they told me that it's normal. Is there any way to check the BAM file to figure out if they made mistakes while converting BAM to VCF?

  • @wakeup9199
    @wakeup9199 2 месяца назад

    Well done, bt still i have doubt!!! So if uploated vcf file in yfull and after that i upload da bam wht is da advantages??

  • @franzbuchel7295
    @franzbuchel7295 2 месяца назад

    Excellent information! Could You explain Epigenetics in an other video and tell what test You would consider for that?

  • @sapandeepsandhu4410
    @sapandeepsandhu4410 3 месяца назад

    SAM File Structure: Header Section: Optional, starts with '@', contains metadata about the sequence and the alignments. Alignment Section: Contains alignment information with each line representing a read. Columns in SAM: QNAME: Query template name. FLAG: Bitwise flag. RNAME: Reference sequence name. POS: 1-based leftmost mapping position. MAPQ: Mapping quality. CIGAR: CIGAR string. RNEXT: Reference name of the mate/next read. PNEXT: Position of the mate/next read. TLEN: Observed template length. SEQ: Segment sequence. QUAL: ASCII of Phred-scaled base quality+33.

  • @sapandeepsandhu4410
    @sapandeepsandhu4410 3 месяца назад

    @SEQ_ID GATTTGGGGTTCAAAGCAGTATCGATCAAATAGTAACTGAGGGTGATGCAG + !''*((((***+))%%%++)(%%%%).1***-+*''))**55CCF>>>>>>CCCCCCC65

  • @sapandeepsandhu4410
    @sapandeepsandhu4410 3 месяца назад

    .FASTQ (Raw Sequence Data) FASTQ is a text-based format for storing both nucleotide sequences and their corresponding quality scores. It is widely used in high-throughput sequencing. File Structure: Header Line: Starts with '@' followed by a sequence identifier. Sequence Line: Contains the nucleotide sequence. Plus Line: Starts with a '+' and may be followed by the same sequence identifier. Quality Line: Contains quality scores for each nucleotide in the sequence, encoded as ASCII characters

  • @phobe645
    @phobe645 3 месяца назад

    Hi Katherine, At this time is it possible to use whole geneome sequencing & whole ecome sequencing in combination at present (5-24)?Your presentation was wonderful on many levels~~thank you!!

    • @KatharineME
      @KatharineME 3 месяца назад

      Hi JC, yes it is possible. The whole exome has sequence data on the coding regions only (2% of genome) while whole genome obviously has sequence data on the whole genome. So the exome data is kind of a subset of the genome data. But you could use them together to increase confidence of variant calls in coding regions. Does that make sense?

    • @JS-de8yi
      @JS-de8yi 3 месяца назад

      Thanks so much for a great answer😅 Katherine